Interactions between Brain-derived Neurotrophic Factor and the TRKB Receptor
نویسندگان
چکیده
منابع مشابه
Interactions between brain-derived neurotrophic factor and the TRKB receptor. Identification of two ligand binding domains in soluble TRKB by affinity separation and chemical cross-linking.
The extracellular domain of the human neurotrophin TRKB receptor expressed in Chinese hamster ovary cells is a highly glycosylated protein, possessing binding ability for brain-derived neurotrophic factor (BDNF). Two distinct ligand binding domains of TRKB were isolated from proteolytic digests of the receptor by affinity separation on immobilized BDNF. One of these domains consists of amino ac...
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BACKGROUND Vagal nerve stimulation (VNS) has been approved for treatment-resistant depression. Many antidepressants increase expression of brain-derived neurotrophic factor (BDNF) in brain or activate, via phosphorylation, its receptor, TrkB. There have been no studies yet of whether VNS would also cause phosphorylation of TrkB. METHODS Western blot analysis was used to evaluate the phosphory...
متن کاملExpression of the neurotrophin brain-derived neurotrophic factor (BDNF) and of its high-affinity receptor TrkB
factor (BDNF) and of its high-affinity receptor TrkB have previously been described in the avian embryo. During early embryonic development, BDNF mRNA expression was identified by reverse transcriptase/polymerase chain reaction (RT-PCR) beginning at stage 1 (3–4 h of embryo incubation), while trkB expression was not detected until the eight-somite stage (29 h) (Baig and Khan, 1996). These obser...
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The expression of brain derived neurotrophic factor (BDNF) and its preferred receptor (TrkB) in rat retinal ganglion cells (RGCs) have been determined in the present study. To identify RGCs retrograde labelling was performed with fluorogold (FG). Subsequently, retinas were immunostained with antibodies to BDNF and TrkB. We found that all RGCs labelled with FG express both BDNF and its preferred...
متن کاملBrain-derived neurotrophic factor receptor TrkB exists as a preformed dimer in living cells
BACKGROUND Neurotrophins (NTs) and their receptors play crucial roles in the development, functions and maintenance of nervous systems. It is widely believed that NT-induced dimerization of the receptors initiates the transmembrane signaling. However, it is still controversial whether the receptor molecule has a monomeric or dimeric structure on the cell surface before its ligand binding. FIN...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1997
ISSN: 0021-9258
DOI: 10.1074/jbc.272.40.25296